I. Gene Cloning
Gene Mutagenesis
siRNA and miRNA
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification

PCR Cloning and Gene Subcloning

NorClone's PCR cloning and gene subcloning services are highly flexible and can meet the requirements of any project. Our gene cloning and subcloning services include:

   1) Cloning the target gene to meet your specific research needs.

   2) Engineering an epitope-tag (i.e., His, HA, Myc, GST, FLAG, ...).

   3) Retrofitting an existing clone into a more desirable vector.

   4) Designing linkers/adapters.

You provide:

   1) 2~5 g of the target gene in a plasmid and/or 2) the gene sequence file

       so it can be generated in our laboratory.

You will receive:

   1) 5 g of cloned plasmid construct.

   2) Stab culture of E. coli carrying the plasmid construct.

   3) Project report including plasmid map and/or sequencing results.

Turnaround Time:

   Approximately 1~2 weeks.

Please contact us for a detailed quote or for more information.


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