Rapid Amplification of cDNA Ends - 5' RACE & 3' RACE
Obtaining a full-length cDNA is one of the important, and often one of the difficult steps in gene hunting and analysis.
amplification of cDNA ends (RACE) technology has been widely used for
discovering the missing portions of cDNA clones.
To expand the utility of RACE technology, NorClone has made
improvements, especially on 5’ RACE. We have developed Power-RACE
protocol. Our 5’ and 3’ Power-RACE services include:
Design the primers
based on sequence provided by you.
Perform 5’ and 3’ Power-RACE reactions.
Sequence the 5’ and 3’ Power-RACE PCR products.
Perform end-to-end PCR.
Clone the full-length cDNA into a TA cloning vector or vector of your
small known cDNA sequence.
Total RNA materials.
1) 5 µg
2) Stab culture of E. coli
the full-length cDNA
3) Project report
us for a detailed quote or for more information.