I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




 

Knockout and Knockin Vector Construction



NorClone provides guaranteed cloning and construction of different types of gene targeting vectors for creating Conventional (Fig. 1), Conditional (Fig. 2), and Conventional/Conditional (Fig. 3) knockout mice and knockin/point mutations (Fig. 4) mice. These services generally include sequence analysis of the genomic structure and exon/intron boundaries of the gene of interest; PCR amplification and/or enzyme digestion; bacterial transformation; plasmid isolation; and confirmation of the desired gene-targeting vector.
 

Genomic DNA of the gene of interest can be provided by customers or prepared by NorClone.

Please contact us for a detailed quote or for more information.


 

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