I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




Isolation of Gene Isoforms



NorClone provides gene isoform RT-PCR service to discover new variants in different tissues and cell cultures. This service includes:

   1) The starting materials can be tissue, cell culture, total RNA or mRNA.
   2) Reverse transcriptase and PCR amplification.
   3) Cloning of the isolated gene isoform into an appropriate vector.
   4) Verification of the gene isoform by sequencing.

Customers will rec
eive:
 

   1) 2~5 g of the isoform plasmid.

   2) Stab culture of E. coli carrying the isoform plasmid.

   3) Sequencing chromatogram and text files.

Please contact us for a detailed quote or for more information.

 

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