I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




CRISPR/Cas9 Genome Editing


 

The precision RNA-guided genome editing by CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple system. This system requires the co- expression of a Cas9 protein with a guide RNA vector. The Cas9 protein is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The cleavage site is within the target sequence 3 bases upstream of the protospacer-adjacent-motif (PAM - the sequence NGG). Then, the double-strand break is repaired by homologous recombination with the modified genome template from the rescue donor vector. In this way, we can generate insertions, deletion, point mutant, in-frame GFP fusions, or any other modification.

 

Custom Services

Reference Price

Target sequence design and cloning into CRISPR/Cas9 vectors

                $125.00 Cdn

                $99.00 US

Donor vector construction with a predesigned cassette

                $950.00 Cdn

                $750.00 US

Donor vector of your own design Gene synthesis cost

                $0.60/bp Cdn

                $0.45/bp US

 

Please contact us for a detailed quote or for more information.

 

 

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