I. Gene Cloning
Gene Mutagenesis
siRNA and miRNA
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification

CRISPR/Cas9 Genome Editing


The precision RNA-guided genome editing by CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple system. This system requires the co- expression of a Cas9 protein with a guide RNA vector. The Cas9 protein is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The cleavage site is within the target sequence 3 bases upstream of the protospacer-adjacent-motif (PAM - the sequence NGG). Then, the double-strand break is repaired by homologous recombination with the modified genome template from the rescue donor vector. In this way, we can generate insertions, deletion, point mutant, in-frame GFP fusions, or any other modification.


Custom Services

Reference Price

Target sequence design and cloning into CRISPR/Cas9 vectors

                $199.00 Cdn

                $169.00 US

Donor vector construction with a predesigned cassette

                $950.00 Cdn

                $750.00 US

Donor vector of your own design gene synthesis

                $0.60/bp Cdn

                $0.45/bp US


Please contact us for a detailed quote or for more information.



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