The precision RNA-guided genome editing by CRISPR/Cas9 allows for specific genome disruption and replacement in a flexible and simple system. This system requires the co- expression of a Cas9 protein with a guide RNA vector. The Cas9 protein is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. The cleavage site is within the target sequence 3 bases upstream of the protospacer-adjacent-motif (PAM - the sequence NGG). Then, the double-strand break is repaired by homologous recombination with the modified genome template from the rescue donor vector. In this way, we can generate insertions, deletion, point mutant, in-frame GFP fusions, or any other modification.