I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




Site-Directed Mutagenesis Service



NorClone provides site-directed mutagenesis service quickly and economically to introduce single or multiple mutations (substitutions, insertions, deletions, or truncations) into existing DNA sequenceThese services include:
 

   1) Design, synthesis, and purification of oligo nucleotide primers.

   2) Mutagenesis reaction, enzyme digestion and/or ligation.

   3) Bacterial transformation.

   4) Plasmid isolation.

   5) Confirmation of the desired mutation by DNA sequencing.

You provide:

   1) 2~5 g of the target gene in a plasmid.

   2) Sequence of the target gene with a list of the bases to be mutated.

You will receive:

   1) 2~5 g of mutant plasmid.

   2) Stab culture of E. coli carrying the mutant plasmid.

   3) Sequencing chromatogram and text files.

Turnaround Time:

   Approximately 1~2 weeks.

 

Prices for mutagenesis from customer-provided Templates:

 Single or multiple mutations

     $199.00 Cdn

     $169.00 US

 

Please contact us for a detailed quote or for more information.

 

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