I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




 

RNAi Substractes - shRNA, siRNA and miRNA



RNA interference (RNAi) is a gene-silencing process in which RNA molecules inhibit gene expression by targeting mRNA. The key RNAi molecules regulating this process are small interfering RNAs (siRNA), microRNAs (miRNA) and short hairpin RNAs (shRNA).

RNAi molecules of 19 to 23 nucleotides are especially effective. And the efficiency of gene suppression is primarily dependent on the portion of the gene sequence targeted. Our services include:

   1)
Identification of RNAi target sequence.
  
2)
Synthesis of the RNAi insert.
   3) Cloning the insert into the viral or non-viral delivery
vector systems.
   4) Confirmation of the RNAi construct by sequencing.


Customers will receive:

   1) Desired RNAi construct DNA.
  
2) Stab culture of E. coli carrying the RNAi construct.
   3) Sequencing chromatogram data.

Turnaround Time:

   Approximately 1~2 weeks.

Please contact us for a detailed quote or for more information.

 

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