Yeast Two-Hybrid Screen Service

The Yeast Two-Hybrid (Y2H) system has been very well utilized to study protein-protein interactions since its introduction in 1987. NorClone provides the Y2H screening services to identify novel protein interactions, to confirm putative interactions, and to define interacting domains. 

Our services include:

   1) Cloning the bait target into Y2H vector and sequencing. It can be a
       short (truncated) or full length gene.

   2) Testing bait for self-activation and toxicity.

   3) Library screening and interaction validation.

   4) Isolating and sequencing positive clones.

You provide either:

   1) 2~5 µg of the bait target gene in a plasmid or 2) the gene sequence file

       so it can be generated in our laboratory.

You will receive:

   1) List of positive clones and sequences (up to 20 clones).

   2) 2~5 µg of plasmid DNA of each positive clone.

Turnaround Time:

   Approximately 3 months.

Please contact us for a detailed quote or for more information.