I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 





Yeast Two-Hybrid Screen Service



The Yeast Two-Hybrid (Y2H) system has been very well utilized to study protein-protein interactions since its introduction in 1987. NorClone provides the Y2H screening services to identify novel protein interactions, to confirm putative interactions, and to define interacting domains. 

Our services include:

   1) Cloning the bait target into Y2H vector and sequencing. It can be a
       short (truncated) or full length gene.

   2) Testing bait for self-activation and toxicity.

   3) Library screening and interaction validation.

   4) Isolating and sequencing positive clones.

You provide either:

   1) 2~5 g of the bait target gene in a plasmid or 2) the gene sequence file

       so it can be generated in our laboratory.

You will receive:

   1) List of positive clones and sequences (up to 20 clones).

   2) 2~5 g of plasmid DNA of each positive clone.

Turnaround Time:

   Approximately 3 months.

Please contact us for a detailed quote or for more information.

 

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