I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




Protein Expression and Purification



NorClone provides recombinant protein expression service to advance and optimize research for customers. These services generally include:

   1) Cloning a gene of interest into a bacterial expression vector if customer 
       does not have it.
   2) Expression screening for 3~5 individual bacterial colonies in a small-scale
       induction experiment.
   3) Optimization of expression by doing a time course of induction, altering
       induction temperature or screening growth media.
   4) Large-scale bacterial culture (one liter) for protein production.
   5) Protein purification using Ni-NTA (for 6xHIS-tagged proteins), glutathione
       (for GST-tagged proteins), or amylose (for MBP-tagged proteins) beads.
   6) QC analysis using SDS-PAGE.

The purified protein and SDS-PAGE gel photo will be delivered in approximately 3 weeks. 


Please contact us for a detailed quote or for more information.

 

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