I. Gene Cloning
Subcloning
Gene Mutagenesis
siRNA and miRNA
CRISPR Cas9
Knockout and Knockin Vector Construction
II. Gene Discovery
Library Construction
Library Screening
5' and 3' RACE
Gene Synthesis
Gene Isoforms
III. Protein-Protein Interaction
Yeast 2 Hybrid Screen
Protein Expression
IV. Cell Culture and Recombinant Virus
Stable Cell Line
Virus Production
V. Molecular Diagnostics
Mouse Genotyping
Bacteria and Fungi Identification
 




Gene Synthesis



NorCone provides guaranteed DNA and gene synthesis service with 100% accuracy. This service generally includes:
 

   1) Sequence analysis (if required). We can codon-optimize customer's
       sequence for a special organism. Based on a protein sequence, we can
       reverse translate it and optimize the DNA sequence for the organism of
       customer's choice.
   2) Synthesis of gene and cloning into a standard vector (pBluescript) or 
       customer's vector.

   3) 100% fidelity guaranteed: sequencing data including chromatogram
       will be provided.

Customers will receive:

 

   1) 5 g of plasmid containing synthesized gene.
  
2) Stab culture of E. coli carrying the plasmid;
  
3) Sequencing chromatogram printout.
 

The turnaround time depends on the gene size. For genes up to 1 kb, the average time is 14 business days.

Please contact us for a detailed quote or for more information.

 

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